Part:BBa_K2751015:Design

Human DKK1 promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1800
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1895
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1247
  Illegal SapI site found at 1674
  

Design Notes

We used PCR technique to amplify a segment of human chromosome 10 (from 52311944 to 52314294). The estimated length of our DKK1 promoter segment is around 2356bp.

Source

DKK1 promoter is amplified by conducting PCR on HEK293 genome.